![]() Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins. They are disposable, pre-assembled stacks that come with an integrated preactivated PVDF or nitrocellulose transfer membrane for dry blotting of proteins from polyacrylamide gels. The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. The regular size (13 cm x 8.3 cm) can be used for all Invitrogen midi gels (or other midi gel types), or two Invitrogen mini gels (or other mini gel types), and E-PAGE 48 or 96 gels. The mini size (8 cm x 8 cm) can be used for all Invitrogen precast and handcast mini gels (or other mini gel types). The protein binding capacity is 240 µg/cm 2. The proteins bind to the membrane due to hydrophobic interactions. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The protein binding capacity is 209 µg/cm 2. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The nitrocellulose membrane (0.2 µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. Each transfer stack contains a copper coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. Electrode plates are fully separated to prevent arching or damage.IBlot and iBlot 2 Transfer Stacks are consumable stacks with integrated PVDF or nitrocellulose transfer membranes for dry blotting of proteins. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes - typically only a few millilitres of buffer are required per transfer. Each unit is compatible with their respective omniPAGE vertical gel system. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes.
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